11081-1-AP

UCP2 antibody from Invitrogen Antibodies

BMIQ4, SLC25A8

Western blot: Supportive data in Antibodypedia

Immunohistochemistry: Supportive data in Antibodypedia

Other assay: Supportive data in Antibodypedia

Provider product page for 11081-1-AP

Antibody data

Antibody Data
References [0]
Comments [0]
Validations
Western blot [7]
Immunohistochemistry [13]
Other assay [1]

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Product number: 11081-1-AP
Provider: Invitrogen Antibodies
Product name: UCP2 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - 11081-1-AP
Antibody type: Polyclonal
Antigen: Other
Description: This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein. This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour. It is strongly recommended that fresh-made lysate be used for western blot assays.
Concentration: 0.2 mg/mL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human ovary tissue slide using 11081-1-AP (UCP2 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human skeletal muscle using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human skeletal muscle using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human ovary tissue slide using 11081-1-AP (UCP2 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using (Product # 11081-1-AP) (UCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).