Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- UM800031CF - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FCER2 Monoclonal Antibody (UMAB101), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB101
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Jurkat cells using anti-CD23 mouse monoclonal antibody (UM800031, green, 1:100). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3 min using pressure chamber/cooker. The image is a composite of tonsils which show strong membranous and cytoplasmic staining however each tonsil has a unique expression pattern in the inner and outer germinal center.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human spleen using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3 min using pressure chamber/cooker. The image is a composite of spleens which show strong membranous and cytoplasmic staining white pulp.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD23 clone UMAB101 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM800031)(1:500).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-FCER2 (CD23) clone UMAB101 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM800031)(1:500).