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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-77995 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Histone H2A.X (Ser139) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: HCT116, HCT116 (30 µM cisplatin treatment for 24 hr), HCT116 (UVB treatment 50mJ for 2hr), HCT116 (UVB treatment 50mJ for 6hr), NIH-3T3 (30µM cisplatin treatment for 24hr), PC-12 (30uM cisplatin treatment for 24hr)
- Concentration
- 0.16 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Histone H2AX (Ser139) in A) NIH-3T3 whole cell lysate (untreated), B) NIH-3T3 whole cell lysate (30µM cisplatin treatment for 24hr) using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with Phospho-Histone H2AX (Ser139) polyclonal antibody (Product # PA5-77995) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Histone H2AX (Ser139) in untreated (-) and treated (+) PC-12 whole cell extracts using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with Phospho-Histone H2AX (Ser139) polyclonal antibody (Product # PA5-77995) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A. X (phospho Ser139) antibody detects H2AFX protein by western blot analysis. A. 30 µg HCT116 whole cell lysate/extract (untreated for 2hr). B. 30 µg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr). C. 30 µg HCT116 whole cell lysate/extract (untreated for 6hr). D. 30 µg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr).15% SDS-PAGE. Histone H2A. X (phospho Ser139) antibody Phospho-Histone H2A. X (Ser139) Polyclonal Antibody (Product # PA5-77995) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A. X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract (untreated). B. 30 µg NIH-3T3 whole cell lysate/extract (30 µM cisplatin treatment for 24hr).15% SDS-PAGE. Histone H2A. X (phospho Ser139) antibody Phospho-Histone H2A. X (Ser139) Polyclonal Antibody (Product # PA5-77995) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Phospho-Histone H2A.X (Ser139) was performed by separating 30 µg of untreated (–) and treated (+) HCT-116 whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Phospho-Histone H2A.X (Ser139) Polyclonal Antibody (Product # PA5-77995) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Phospho-Histone H2A.X (Ser139) was performed by separating 30 µg of untreated (–) and treated (+) PC-12 whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Phospho-Histone H2A.X (Ser139) Polyclonal Antibody (Product # PA5-77995) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Phospho-Histone-H2A.X-(Ser139) was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Phospho-Histone H2A.X (Ser139) Polyclonal Antibody (Product # PA5-77995) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-Histone H2A. X (Ser139) Polyclonal Antibody detects Histone H2A. XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Histone H2A. XS139ph (phospho Ser139) stained by Phospho-Histone H2A. X (Ser139) Polyclonal Antibody (Product # PA5-77995) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
