Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 935702 - Provider product page
- Provider
- BioLegend
- Product name
- Purified anti-RPS6 Phospho (Ser244)
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- A18024A
- Vial size
- 100 µg
- Concentration
- 0.50 mg/mL
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysates (15 µg protein) from serum-starved SR cells treated without (-) or with (+) 160 nM PMA for 30 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2500 dilution) of purified anti-RPS6 Phospho (Ser244) antibody (clone A18024A). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using a pan RPS6 antibody and Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: molecular weight ladder.
- Conjugate
- Unconjugated